Highly optimized solution for fast and efficient cDNA synthesis and qPCR amplification in a single tube. Supporting up to 7 targets, it is ideal for demanding workflows and enables rapid, sensitive detection of RNA targets with unparalleled precision.
Details
NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) is a cutting-edge, highly optimized master mix engineered for superior performance in one-step reverse transcription and real-time quantitative PCR (RT-qPCR).
Designed to enable cDNA synthesis and qPCR amplification in a single tube, this master mix ensures streamlined workflows, reduced handling steps, and minimal contamination risk. The formulation of NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) supports the rapid and sensitive detection of RNA targets with unparalleled precision. With a faster cDNA synthesis step of only 1 minute at 60 °C, combined with an accelerated qPCR cycling protocol, this mix significantly reduces assay time while maintaining exceptional sensitivity and reproducibility. Capable of amplifying and detecting up to 7 targets simultaneously in a single reaction, NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) delivers unmatched multiplexing capacity, ideal for high-throughput applications, viral and bacterial pathogen detection, and complex assays.
This master mix is particularly well-suited for demanding molecular biology workflows requiring enhanced performance in the presence of inhibitors commonly found in clinical and environmental samples.
NZY Ultra Multiplex One-step RT-qPCR Probe Master Mix (2x) is formulated as a concentrated 2× solution containing all necessary components for robust,