Provides all the required components to efficiently synthesize first-strand cDNA from an RNA template, offering flexible priming options.
Details
Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack is a versatile kit designed for efficient, fast and reliable first-strand cDNA synthesis. It offers a comprehensive solution, including:
High-Performance Reverse Transcriptase: Supreme NZY Reverse Transcriptase 1 kU/µL, known for its stability, accuracy, and ability to synthesize full-length cDNA;
Optimized Buffer: 10x Reaction Buffer specifically formulated for the reverse transcriptase, ensuring optimal enzyme performance;
RNase Inhibitor: Supreme NZY Ribonuclease Inhibitor is included to protect RNA from degradation;
dNTPs: A balanced mix of dNTPs for complete cDNA synthesis;
Primers: Both random hexamers and oligo(dT) 18 primers are provided for flexible priming options;
RNase H: For RNA degradation in cDNA-RNA hybrids, essential for downstream applications.
The pack is suitable for a wide range of RNA samples and total RNA concentrations, making it adaptable to various experimental needs. The produced cDNA can be used for various downstream applications, including real-time PCR, standard PCR, and microarrays. Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack offers a streamlined and efficient workflow for cDNA synthesis, providing researchers with a reliable and convenient tool for their molecular biology experiments.
Components
Supreme NZY Reverse Transcriptase 1 kU/µL
Dilution Buffer for Supreme Reverse Transcriptase
Reaction Buffer 10x for Supreme NZY Reverse Transcriptase
Supreme NZY Ribonuclease Inhibitor
dNTP Mix (10mM each)