Solution reagent kit E-Z 96™

for researchfor RNA extraction

The E-Z 96™ Total RNA Kit is designed for isolation of total cellular RNA from up to 5 x 105 cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilizing HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High-quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96™ Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications. Specifications Not for use in diagnostic procedures. FEATURES - SPECIFICATIONS Downstream Application - PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification Elution Volume - 50-75 μL Starting Material - Culture cells Starting Amount - up to 5x105 cells Processing Mode - Manual, centrifugation/vacuum Throughput - 96 samples per run RNA Binding technology - Silica membrane 96-well