Magnetic bead-based reagent kit Mag-Bind®

for researchfor PCRNGS

Omega Bio-tek’s Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups. No protocol change against major competitor Read lengths averaging over 800 bps (Min Phred 20) Manual or adjustable to automated liquid handlers Significant cost savings compared to sephadex-based clean ups 96- or 384-well formats Specifications For Research Use Only. Not for use in diagnostic procedures. FEATURES SPECIFICATIONS Downstream application Sanger Sequencing, Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting Elution volume 40 µL or above Starting material ABI Big Dye Chemistry Cycle Sequencing Reactions Starting amount 5 - 20 µL DNA recovery Sequencing reads start at 40 nt Processing mode Automated; Manual Throughput 96-384 samples per run