Agarose gel reagent kit E.Z.N.A.®

for PCRfor DNA extractionfor DNA sequencing

The E.Z.N.A.® Gel Extraction kit uses the proprietary HiBind® spin-column technology to purify DNA fragments ranging from 100 bp to 10 kb from all grades of agarose gels with high recovery (> 80%). The kit uses a specialized binding buffer system that not only dissolves the gel slice and binds to the spin column but also includes a pH indicator for a visual representation of optimal pH for DNA binding. The bind step is followed by three rapid wash steps and DNA is eluted with deionized water or elution buffer. Purified DNA is ready for a variety of downstream applications such as ligations, PCR amplification, restriction enzyme digestion, cloning, and various labeling reactions. Rapid – DNA recovery from an agarose gel in 15 min Specialized buffer system – Visual determination of optimum DNA binding for higher yields Safe – No phenol/chloroform extractions Versatile – Spin and vacuum formats available High-quality – DNA is suitable for a variety of downstream applications Specifications Not for use in diagnostic procedures. FEATURES SPECIFICATIONS Downstream application Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting Elution volume 30-50 µL Starting material Agrose gel slice Starting amount Up to 25 µg DNA DNA recovered >90% recovery, 70 bp to 20 kb Processing mode Manual, centrifugation/vacuum Throughput 1-12 DNA binding technology Silica mini spin column Binding capacity 25 μg Processing time <10 minutes