Clean up DNA in low elution volume from PCR reactions using spin columns
Overview
Modified from the E.Z.N.A.® Cycle Pure Kit, the E.Z.N.A.® MicroElute Cycle Pure Kit is specially designed to purify PCR samples with a small elution volume of 10-15 µL. Highly concentrated DNA eluates are ready to use in all subsequent applications.
Elution volumes – 10-15µl
Rapid – Purification of PCR products in 10 minutes
Convenient – No phenol/chloroform extractions
Versatile – Spin and vacuum formats available
High-quality – DNA is suitable for downstream application
Specifications
For Research Use Only. Not for use in diagnostic procedures.
FEATURES SPECIFICATIONS
Downstream application Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume 10-15 µL
Starting material PCR reaction
Starting amount Up to 10 µg DNA
DNA recovered >90% recovery, 70 bp to 20 kb
Processing mode Manual, centrifugation/vacuum
Throughput 1
DNA binding technology Silica MicroElute spin column
Binding capacity 10 μg
Processing time <15 minutes
Special note Low elution volume