Solution reagent kit E.Z.N.A.®

for researchfor PCRfor DNA sequencing

Isolate up to 25 µg plasmid DNA from 1-5 mL bacterial cultures using mini spin columns Overview The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing. The E.Z.N.A.® Plasmid Mini Kit is available in two formats – Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols. Rapid – Purification of plasmid DNA in less than 30 minutes Safe – No Phenol/chloroform extractions Versatile – Spin and vacuum formats available High-quality – DNA is suitable for a variety of downstream applications Specifications For Research Use Only. Not for use in diagnostic procedures. FEATURESSPECIFICATIONS Downstream Application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. Starting material 1-5 mL LB culture Plasmid type High-copy, low-copy, cosmid DNA Processing mode Manual (centrifugation or vacuum) Throughput 1-24 DNA binding technology Silica Mini Spin Column Lysate clearance method Centrifugation Processing time <30 minutes