Solution reagent kit E.Z.N.A.®

for researchfor PCRfor DNA purification

Isolate up to 1.2 mg plasmid DNA from 50-200 mL bacterial cultures using maxi spin columns Overview The E.Z.N.A.® Plasmid Maxi Kit utilizes the convenient spin-column format to deliver high-quality plasmid DNA following a simple “bind-wash-elute” procedure without need for expensive accessories. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications. Speed – Purification of plasmid DNA in < 60 minutes Safety – No phenol/chloroform extractions Versatile – Spin and vacuum formats available High quality – DNA is suitable for a variety of downstream applications Specifications For Research Use Only. Not for use in diagnostic procedures. FEATURES SPECIFICATIONS Downstream Application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc. Starting material 50-200 mL LB culture with OD600 between 2 and 3; or equivalent Plasmid type High-copy, low-copy, cosmid DNA Processing mode Manual (centrifugation or vacuum) Throughput 1 - 24 DNA binding technology Silica Maxi Spin Column