Solution reagent kit E-Z 96™

for researchfor DNA sequencingfor molecular biology

Isolate plasmid DNA from bacterial cultures in 96-well plate format Overview The E-Z 96® FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96® Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers. Clear lysate with E-Z 96® Lysate Clearance Plate No resin, slurries, or alcohol precipitation High-purity plasmid DNA in less than 1 hour Reproducible yields of molecular biology grade plasmid DNA Fast, easy and convenient protocols Specifications Not for use in diagnostic procedures. FEATURES SPECIFICATION Downstream Application Cloning; sanger sequencing etc. Starting material Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent Plasmid type High-copy, low-copy, cosmid DNA Processing mode 96-well, centrifugation or vacuum Throughput 96 samples per run DNA binding technology Silica 96-well plate Lysate clearance method Centrifugation or vacuum with lysate clearnce plate Processing time <1 hour Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number Special note Lysate clearance plate for fast processing