Solution reagent kit E.Z.N.A.®

for researchfor DNA sequencingfor nucleic acids

Isolate plasmid DNA from up to 15 mL bacterial cultures using spin mini columns Overview This second generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing. Rapid – Purification of plasmid DNA in 30 minutes or less Safe – No phenol/chloroform extractions Versatile – Spin and vacuum formats available High-quality – DNA is suitable for downstream applications Specifications For Research Use Only. Not for use in diagnostic procedures. FEATURES SPECIFICATIONS Downstream Application Cloning; sanger sequencing etc. Starting material 5-15 mL LB culture with OD600 between 2 and 3; or equivalent Plasmid type High-copy, low-copy, cosmid DNA Processing mode Manual, centrifugation or vacuum Throughput 1 - 24 DNA binding technology Silica Mini Spin Column II (2x capacity of mini spin column) Lysate clearance method Centrifugation Processing time <30 minutes Yield 40-70 µg for high copy-number; 1-15 µg for low copy-number