The Opentrons Protein Purification Workstation is an open-source liquid handling system that fully automates small scale protein purification, proteomics sample processing, enzymatic and chemical lysis, and general liquid handling and reagent distribution for various chemical assays at a fraction of current market cost.
This Opentrons Protein Purification Workstation uses the Biotage PhyTip® pipette tips to perform dual flow chromatography-based protein purification.
The following Biotage PhyTip Columns for the OT-2 are available
C18 300Å
C18 90Å
C4 300Å
CaptureSelect IgG-Fc Hu
CaptureSelect IgG-Fc
CaptureSelect KappaXL
CaptureSelect IgG-Fc-ms
Glutathione
IMAC
Normal Phase 1
POROS CaptureSelect IgM
ProPlus & ProPlus LX(MabSelect Sure™)
Protein A
Protein G
Streptavidin
Strong Anion
Strong Cation
Weak Anion
This workflow can be used for the following applications:
Screening and optimization of purification conditions
High-throughput protein purification for downstream applications
Capture of macromolecular complexes (protein-protein, protein-DNA)
Protein purification, screening and optimization
Enzymatic and chemical lysis
General purpose liquid handling
Reagent distribution
Protein Purification Workstation Materials (Dual Flow Chromatography-based)
Each Opentrons Protein Purification Workstation (Dual Flow Chromatography-based ) is custom configured with the hardware, consumables and protocol unique to your protein purification assay. You’ll also enjoy free access to the Opentrons Protocol Designer, Python API and Protocol Library.