DNA library preparation laboratory workstation

automatedfor sequencingNGS

Next-generation sequencing (NGS) is the basis of many vitally important areas of life science, but preparing samples for sequencing is a laborious, meticulous process. The Opentrons Automated NGS Library Prep Workstation of robotics and high-quality reagents accompanied with a verified protocol, enables nearly any lab to start automating NGS library preparation end-to-end. After placing your samples on the OT-2 lab robot, it will carry out enzymatic preparation, ligation and set up amplifications using the Temperature Module to keep your reagents cooled. Then, it will use the automated on-deck Thermocycler Module to run PCR steps and perform bead-based cleanups on the Magnetic Module. This Opentrons workstation includes everything you need to start automating NGS library prep in your lab; the OT-2 robot with Thermocycler Module, Temperature Module, Magnetic Module, labware, Swift Bioscience library prep kit, and Omega Bio-tek magnetic beads. This workstation can be used for the following applications: Whole genome sequencing (WGS) DNA sequencing Metagenomic sequencing Long-range PCR amplicons RNA-Seq starting with full length, double stranded cDNA input Detection of germline inherited SNVs and Indels Copy number variation detection Hybridization capture of relevant genomic regions (i.e., the exome) or transcripts of interest Performance Accuracy: 15% at 1uL Precision: 5% at 1uL Throughput: Fast fill a 96-well plate in 22 seconds Updates Supports Ethernet or USB connection (external dongle provided) 4 USB ports available to support connection to multiple modules Easy access to electronics via external PCBA enclosure