Reverse transcriptase reagent PB90.21 series
RNase inhibitordiagnosticlaboratory

reverse transcriptase reagent
reverse transcriptase reagent
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Characteristics

Type
reverse transcriptase, RNase inhibitor
Applications
diagnostic, laboratory, for qPCR, RT-qPCR
Other characteristics
hot start, glycerol-free
Storage temperature

Max.: -15 °C
(5 °F)

Min.: -30 °C
(-22 °F)

Description

Fast air-drying protocol Superior low copy number detection Unaffected by drying & reconstitution Air-Dryable Probe 1-Step Mix is a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA and DNA sequences. Designed for rapid air-drying, this qPCR mix enables dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation. An optimal blend of excipients ensures reliable air-drying, without loss of activity. Features Unbiased, sensitive detection of both DNA and RNA targets, 4 copies per reaction Complete 1-step RT-qPCR mix, single tube format Easy reaction set up, add only primers & probes before drying Rapid air-drying protocol, 80-90 min drying time Same high performance before & after drying Room temperature storage and shipping of dried reactions Increased sample volume input More Information The Air-Dryable Probe 1-Step Mix includes a glycerol-free 4x qPCR mix containing hot start Taq polymerase, dNTPs, MgCl2 and a blend of excipients to ensure reliable air-drying, without loss of activity. The mastermix also contains a novel version of UltraScript Reverse Transcriptase, modified to increase stability upon drying, and our RiboShield™ RNase inhibitor, so the final blend is suitable both for qPCR detection of DNA and 1-Step RT-qPCR for detection of RNA targets. Designed for rapid air-drying, Air-Dryable Probe 1-Step Mix is intended for use in in-vitro diagnostic kit development (IVD) but can also be used for anyone wishing to benefit from the advantages of dried (RT-)qPCR reactions.A

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