Solution reagent kit Illumina®

dilution bufferNGShot start

The kit uses qPCR to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data. Features Uses qPCR to accurately and rapidly quantify a library prior to sequencing Gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content Uses a single extension time for all libraries Allows specific quantification of only DNA molecules that can be sequenced by NGS Uses antibody-mediated hot start technology to ensure all reactions start simultaneously Compatible with all Illumina® instruments and qPCR platforms Suitable for manual and automated workflows Available as an easy-to-see blue qPCR mix Easily calculate library concentration with the online NGSBIO Library Quantification Tool More Information The NGSBIO Library Quant Kit offers a reliable qPCR-based method for the quantification of libraries prepared for Illumina® NGS systems. The kit includes 5 DNA standards, primers specific to the P5 and P7 Illumina® adapter sequences and qPCRBIO SyGreen Mix. The advanced qPCR buffer system has been developed using our high-throughput smart screen technology to ensure efficient amplification of all your libraries, including those that are GC or AT-rich. The kit is also supplied with a convenient library dilution buffer. Accurate Library Quantification qPCR is considered the best method for quantifying NGS libraries as it only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation.