Enzyme reagent kit VeriFi™

DNA polymerasedyefor PCR

Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates. Features AptaLock™ hot start technology for maximised sensitivity and specificity Greater success with long and/or GC or AT-rich templates (17.5 kb and over) High temperature cycling – up to 100°C denaturation to better separate GC-rich sequences 100x higher fidelity than Taq DNA polymerase Room temperature setup Reaction mix stability for up to 24 hours both before and after PCR run Generates blunt-end PCR products Also available as a 2x ready mix with the option of a red dye for direct gel loading More Information VeriFi™ Hot Start Polymerase is a single enzyme derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Proprietary mutations improve DNA binding and increase processivity when compared to its native form, resulting in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. VeriFi™ Hot Start Polymerase is able to amplify eukaryotic genomic templates in excess of 17.5 kb, and longer for simpler DNA templates. Extension times of 30 s per kb are recommended for most applications. PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’-3’ polymerase activity of the enzyme below 40°C. This unique hot start molecule prevents primer dimer formation and non-specific amplification to maximise the sensitivity and specificity of your PCR.