Isothermal amplification reagent kit IsoFast™ Bst

enzymeDNA polymerasedye

The kit contains IsoFast™ Bst Polymerase, providing strong strand displacement capabilities, together with the highly active modified MMLV RTase Go. Features Validated for qualitative detection of SARS-CoV-2 nucleic acid Includes strand-displacing IsoFast™ Bst Polymerase in a 2x mix format Supplied with RTase Go plus RNase inhibitor Reaction is carried out at 65˚C Gives rapid and consistent amplification across a wide range of templates Includes an advanced buffer system for higher yield under difficult conditions Supplied with a fluorescent dye for real-time detection 30 minute protocol More Information IsoFast™ Bst 1-Step Mix is designed for reverse transcription of target RNA and subsequent isothermal amplification in a single tube. Dual enzyme system The kit utilises IsoFast™ Bst Polymerase for its strong strand displacement activity, enabling DNA synthesis at a constant temperature (65˚C) without the need for thermal cycling. Representing the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this portion of the protein catalyses the 5’-3’ synthesis of DNA but does not contain the 5’-3’ exonuclease domain.1 The enzyme is provided in a convenient 2x mix format. Reverse transcription of target RNA is carried out by the thermostable and extremely active RTase Go, which is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.