Isothermal amplification reagent kit IsoFast™ Bst

enzymeDNA polymerasenucleic acid

The enzyme offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification. Features Has strand-displacing 5′-3′ polymerase activity Lacks 5′-3′ exonuclease activity DNA synthesis is performed at a constant temperature Operates over a broad temperature range, with an optimum of 65ºC Gives rapid and consistent amplification across a wide range of templates Supplied with an advanced 2-part buffer system for higher yields under difficult conditions 30 minute protocol Flexible formats with and without fluorescent dye Also available as a 2x mix and as a dual enzyme 1-step system for RNA amplification Glycerol-free enzyme More Information IsoFast™ Bst Polymerase is a recombinant protein expressed in E. coli and represents the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA Polymerase. This portion of the protein catalyses the 5′-3′ synthesis of DNA and has strand displacement activity but does not contain the 5’-3’ exonuclease domain.1 Strong strand displacement Strand displacement refers to the ability of an enzyme to dissociate the hydrogen bond of double stranded template DNA encountered downstream, essentially unzipping the DNA as the complementary strand is synthesised. IsoFast™ Bst Polymerase displays strong strand displacement activity and is suitable for amplification methods including whole genome amplification, multiple displacement amplification and isothermal amplification.