Enzyme reagent kit IsoFast™

DNA polymerasedyeBst DNA polymerase

IsoFast™ Hot Start Bst Colour reagents are colourimetric isothermal amplification enzyme formulations that combine IsoFast™ Hot Start Bst Polymerase with a pH-based dye for rapid positive/negative screening. IsoFast™ Hot Start Bst Polymerase is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. This colourimetric formulation is available both as a preassembled mix, IsoFast™ Hot Start Bst Colour Mix, for direct sample and primer input. Alternatively, IsoFast™ Hot Start Bst Polymerase Colour is available as a separate Bst DNA Polymerase and colour buffer system, which allows full assay customisation and maximum setup flexibility. This isothermal amplification system enables PCR-free, direct, colour-based readout of DNA targets that is ideal for point-of-care diagnostics and field testing. Features: Fast colour readout for positive/negative testing AptaLock™ hot start for ultra-sensitive detection of DNA targets Rapid polymerisation for faster time to results (as little as 10 mins) Detect down to 3 target copies per μL Ideal for both cold and room temperature setup Improved speed and sensitivity for early target detection High activity at a broad range of temperatures from 55-70 °C More Information IsoFast™ Hot Start Bst Polymerase Colour and IsoFast™ Hot Start Bst Colour Mix are the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes in order to meet the demands of molecular diagnostics and research.