DNA polymerase reagent kit qPCRBIO SyGreen Mix

dyefor DNA quantificationfor real-time PCR

qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency. Features Rapid extension rate for early Ct values Market-leading sensitivity – increased limit of detection Specific amplification from complex templates including GC-rich sequences Non-PCR inhibiting intercalating dye Also available as an easy-to-see blue mix Compatible with all standard and fast cycling real-time instruments More Information qPCRBIO SyGreen Mix is designed for fast, highly sensitive and reproducible real-time PCR, with minimal or no optimisation required. The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity. Our 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions. Applications Absolute quantification Relative gene expression analysis High-throughput qPCR from genomic, cDNA and viral sequences Detection of extremely low copy number targets Crude sample PCR