qPCRBIO SyGreen Blue Mix is particularly useful in reducing errors when loading small volume reactions or using white plates.
Features
Easy-to-see blue mix for greater pipetting precision and accuracy
Rapid extension rate for early Ct values
Market-leading sensitivity – increased limit of detection
Specific amplification from complex templates including GC-rich sequences
Non-PCR inhibiting intercalating dye
Compatible with all standard and fast cycling real-time instruments
More Information
Our blue mixes contain a non-reactive dye to improve reaction mix visibility, allowing greater pipetting precision and reduced errors without affecting your real-time PCR performance. qPCRBIO SyGreen Blue Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences, and is able to reliably detect extremely low copy number targets. The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity.
This 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Blue Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.
Applications
Absolute quantification
Relative gene expression analysis
High-throughput qPCR from genomic, cDNA and viral sequences
Detection of extremely low copy number targets
Crude sample PCR