Dye reagent kit qPCRBIO SyGreen Blue Mix
for qPCRfor nucleic acidshot start

dye reagent kit
dye reagent kit
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Characteristics

Type
dye
Applications
for nucleic acids, for qPCR
Other characteristics
hot start
Storage temperature

Max.: -15 °C
(5 °F)

Min.: -30 °C
(-22 °F)

Description

qPCRBIO SyGreen Blue Mix is particularly useful in reducing errors when loading small volume reactions or using white plates. Features Easy-to-see blue mix for greater pipetting precision and accuracy Rapid extension rate for early Ct values Market-leading sensitivity – increased limit of detection Specific amplification from complex templates including GC-rich sequences Non-PCR inhibiting intercalating dye Compatible with all standard and fast cycling real-time instruments More Information Our blue mixes contain a non-reactive dye to improve reaction mix visibility, allowing greater pipetting precision and reduced errors without affecting your real-time PCR performance. qPCRBIO SyGreen Blue Mix can be used to quantify any DNA template including genomic, cDNA and viral sequences, and is able to reliably detect extremely low copy number targets. The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity. This 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Blue Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions. Applications Absolute quantification Relative gene expression analysis High-throughput qPCR from genomic, cDNA and viral sequences Detection of extremely low copy number targets Crude sample PCR

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