Reverse transcriptase reagent kit qPCRBIO Probe 1-Step Go

RNase inhibitorfor real-time PCRfor RNA quantification

The kit is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacon probes. Features Rapid and sensitive detection of RNA viruses including SARS-CoV-2 Rapid extension rate for early Ct values Market-leading sensitivity – increased limit of detection High efficiency in multiplex reactions Advanced RNase inhibitor Antibody-mediated hot start PCR Compatible with all real-time PCR platforms – standard and fast cycling conditions More Information qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and sensitive probe-based real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube. qPCRBIO Probe 1-Step Go is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacons probes. The kit includes a 2x mix and can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of RNA viruses including SARS-CoV-2. The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex.