Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.
Features
50x higher fidelity than Taq DNA polymerase
Increased PCR success rates with longer amplicons
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
Efficient and specific amplification from complex templates including GC rich and AT rich sequences
Stable at 25°C and 37°C for 4 weeks
More Information
The enhanced DNA binding of PCRBIO HiFi Polymerase allows for improved processivity, increasing yield and shortening cycling times (35 cycles of a 5kb amplicon in under 1.5hours). The enhanced efficiency of PCRBIO HiFi Polymerase minimises PCR inhibition, from impure samples such as colony PCR and direct PCR.
PCRBIO HiFi Polymerase uses the latest developments in DNA polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system are room temperature stable for 4 weeks and give superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HiFi Polymerase performs consistently well on a broad range of templates including both GC and AT rich.