Medium reagent MicroSight® MSI

tissuefor nucleic acidsfor cancers

MicroSight® MSI is a simple and fast method for evaluating microsatellite instability (MSI) status of human DNA. The analysis is based on PentaBase’s proprietary INA® technology that enables fast and easy detection of microsatellite instability status by real-time PCR and high-resolution melt (HRM) analysis. MicroSight® MSI requires very little hands-on time and enables evaluation of patient microsatellite status of purified human genomic DNA in less than one and a half hours for fast treatment guidance of cancer patients. MicroSight® MSI can be used for analysis of both paired and unpaired tumour samples. Evaluation of unpaired tumour samples is done by comparison to an included universal reference. Up to 7 unpaired tumour samples can be analysed in each real-time PCR run. Used with BaseTyper 48.4 real-time PCR system Paired and unpaired sample analysis Semi-automatic objective analysis Results in less than 2 hours Minimal hands-on time CE IVD Product Description PCR master mix and oligonucleotide mix for evaluation of MSI status of BAT25, BAT26, NR22 and MONO27, universal reference, positive and negative control Format Ready-to-Use Reactions 16 MicroSight® MSI 1-step HRM analysis is based on Real-Time Polymerase Chain Reaction (RT-PCR) followed by high-resolution melt (HRM) study on a BaseTyper™ 48.4 Quiet HRM Real-Time PCR System. The assay is intended for the qualitative evaluation of microsatellite stability (MSS)/instability (MSI), in human DNA from solid biopsies such as Formalin-Fixed Paraffin-Embedded (FFPE) tissue.