Enzyme reagent EpiDirect® MGMT

for qPCRliquidgenomic DNA

The EpiDirect® MGMT Methylation qPCR Assay represents a new and innovative approach allowing for direct quantification of MGMT promoter methylation by qPCR without prior DNA conversion. The EpiDirect® technology is based on our unique INA® chemistry and includes an EpiPrimer™ oligonucleotide that specifically binds and primes amplification of methylated target DNA. The assay is able to quantify MGMT promoter methylation using standard real-time PCR instruments* in just two hours with as little as 0.5 ng of unconverted input DNA. Standard qPCR instrument procedure No need for prior conversion of DNA Results in less than 2 hours 1.5-3% LoD methylation Minimal hands-on time 0.5-50 ng input DNA CE IVD Product Description PCR master mix and oligonucleotide mix for quantification of MGMT promoter methylation, positive and negative control Format Ready-to-Use Reactions 48 EpiDirect® MGMT Methylation qPCR Assay is a semi-automated real-time Polymerase Chain Reaction (PCR) assay intended for the direct, semi-quantitative analysis of the methylation status of the human DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) gene promoter. The assay is intended for analysis of purified genomic DNA (gDNA) samples that without further pre-treatment can be used with real-time PCR systems for the detection and quantification of the methylation status of the MGMT promoter. Samples comprise Formalin-Fixed Paraffin-Embedded (FFPE) and fresh frozen biopsies and can be prepared using automated platforms or manual workflows.