Buffer solution reagent BaseBlocker™

for molecular biologylyophilized

BaseBlockers™ are short INA® oligonucleotides that are highly modified with intercalating pseudo-nucleotides (IPNs). BaseBlockers™ have extremely high target affinity and specificity which makes them useful for target-specific blocking of PCR amplification. BaseBlocker™ features and advantages include: Reduced false positive PCR assay results Extremely high target affinity and specifity Sequence specific blocking of PCR amplification Particularly useful as part of low frequency SNV detection assays Product Description BaseBlocker™ design (436), 10 nmol delivered (437), 50 nmol or higher delivered (438) Purification HPLC Yield 10/50 nmol or higher delivered Format Lyophilized or dissolved (water/TE buffer) Binary Binding Dynamics BaseBlockers™are otherwise unmodified INA® oligos containing particularly many IPN molecules that provides BaseBlockers™ with an extremely high target affinity and specificity. BaseBlockers™ are furthermore modified to ensure that they do not work as primers. The resulting target vs. off-target binding dynamics of BaseBlockers™ can resemble a binary response (binding vs. no binding) even with single nucleotide variation between target and off-target sequences. Customized BaseBlockers™ can be designed by PentaBase for your specific targets and applications. Reduced Off-target Amplification The extreme sensitivity of BaseBlocksers™ to target sequence variation can be utilised to prevent binding of e.g. allel-specific primers to wild type sequences.