Buffer solution reagent EpiPrimers™

for molecular biologylyophilized

The INA®-based EpiPrimer™ enables direct evaluation of DNA methylation by qPCR without prior conversion of the sample such as chemical bisulfite treatment. Assays based on the EpiPrimer™ technology also require much lower amounts of sample DNA compared to methods using converted DNA. When combined with PentaBase’s HydrolEasy® probe and SuPrimer™, the EpiPrimer™ technology can be used to design powerful customised EpiDirect® qPCR assays that directly measure the methylation frequency of relevant targets. Low sample DNA input requirements Can be customised to specific targets No need for chemical conversion of sample DNA Backbone of EpiDirect® methylation qPCR assays Enables direct qPCR-based quantification of DNA methylation Purification HPLC Yield 10/50 nmol or higher delivered Format dissolved in water/TE buffer Verification Melt analysis of EpiDirect® Anchor using complementary methylated and unmethylated target The EpiPrimer™ is the technological backbone of the EpiDirect® assays and is designed to bind with high affinity to the methylated target and thereby specifically prime direct amplification of methylated DNA vs. non-methylated DNA without prior chemical conversion. The EpiPrimer™ consists of 3 parts: a) an IPN-containing anchor sequence that binds to the methylated target region, b) a starter sequence that primes DNA replication, and c) a loop sequence that is part of the subsequent methyl-unspecific priming. Thus, the anchor and starter sequence prime the initial replication of the methylated DNA while the loop and starter sequence combined work as a primer in the remaining PCR cycles.