Solution reagent HydrolEasy®

for molecular biologylyophilized

The HydrolEasy® probe is an improved alternative to TaqMan®1 and molecular beacon hydrolysis probes. The INA® technology used in HydrolEasy® probes creates a secondary structure in the unbound state that efficiently quenches the fluorescence signal at all temperatures. The result is a very low background fluorescence and consequently higher signal-to-noise ratio compared to standard hydrolysis probes. HydrolEasy® probes are highly suitable for multiplexed real-time PCR applications where multiple probes with the same fluorophore are used. Increased signal-to-noise ratio Improved target affinity and specificity Unbound probe is quenched at all temperatures Available with a wide range of fluorophores and quenchers Ideal for same-color multiplexed real-time PCR applications Flourophores More than 35 fluorophores including PentGreen/Yellow/Orange/Red, CAL Flour, Quasar, ATTO and CYanine – see catalogue for details Purification HPLC Yield 10/50 nmol or higher delivered Verification Melt analysis using complementary target Naturally Quenched By adding hydrophobic IPN molecules close to the ends of the dual-labelled HydrolEasy® probe, the fluorophore and quencher are automatically brought into close proximity due to interactions between IPN molecules. This leads to quenching of the unbound probe at all temperatures. The addition of IPN molecules to HydrolEasy® probes is automatically done by PentaBase when receiving an order and is included in the price. The number of added IPN molecules depends on the desired melting temperature of the probe. The addition of IPN molecules typically adds 4-8°C to the melting temperature.