Combining holography and fluorescence data in one experiment
Minimal light exposure to reduce photocytotoxicity
Small footprint so it fits inside your incubator
Long-term, single-cell and cell population data
Intuitive and guided workflow from setup to analysis
The fluorescence add-on is compatible with the HoloMonitor M4 system. This way, you can tailor the HoloMonitor Live Cell Assays to your needs and add fluorescence to your HoloMonitor system to best fit your research application.
App Suite 4.0 software for fluorescence
The new Fluorescence Capture in App Suite realizes the full potential of HoloMonitor M4FL. You get to combine label-free holography with fluorescence imaging data.
Featured Applications
Illuminate, Analyze and Discover
CELL DEATH
REPORTER GENE EXPRESSION
CO-CULTURE
TRANSFECTION EFFICIENCY
UPTAKE ASSAY
LIVE CELL STAINING
Assay Output
What results do you get with HoloMonitor Fluorescence Capture?
In addition to all the label-free holography cell features to study your cells’ behavior, you can analyze your cells’ fluorescence signal in HoloMonitor’s Single Cell Tracking and Cell Morphology Assay.
Single Cell Tracking screenshot from App Suite showing a graph and green fluorescent cells
Fluorescence Results in the Single Cell Tracking Assay
Morphology screenshot from App Suite showing a dotplot and green fluorescent cells
Fluorescence Results in the Cell Morphology Assay
Technical Specifications
Fluorescence Add-on
1-channel fluorescence - Optimized for EGFP (FITC-Cy2)
Excitation - 470 nm filtered LED, with bandwidth (FWHM): 40 nm
Emission - 525 nm filtered, with bandwidth (FWHM): 50 nm
Exposure time - 1 – 1000 ms