Solution reagent Bright-Dtech™

antibodycell imagingfor Western blot

With Bright-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics. Secondary antibodies aim to detect and visualize a primary antibody that is bound to its protein of interest. Rabbit antibody is often used in applications such as ELISA, Western Blot, IHC, or cell imaging. If you are using a primary antibody produced in rabbits, you will choose as the secondary antibody an anti-rabbit antibody produced in a species other than rabbit. Due to lanthanide’s fingerprinting emission spectrum, Bright-Dtech™ – Nd (Infrared) can be incorporated with other Bright-Dtech™ nanoparticles to achieve multiple detection of biomarkers or proteins in the same biological sample. Before to use Bright-Dtech™, please to check these conditions: > It is strongly recommended not to use PBS buffer Excitation source: 340 nm You can use any peak of the spectra to measure your signal Choosing the right plate reader will allow you to get the best possible results. Each piece of equipment is important. Discover our non-exhaustive list of analysis equipment compatible with our technology: Time-Resolved Fluorescence microplate reader (TRF or HTRF®) Lateral flow reader ADDITIONAL INFORMATION WEIGHT - 0.7 kg DIMENSIONS - 14 × 15 × 11 cm VOLUME - 250 µl, 500 µl, 1 ml, 1,5 ml, 2 x 1,5 ml, 3 x 1,5 ml, 4 x 1,5 ml CONCENTRATION - 10 nM COLOR - Infrared COUPLED TO - Anti-Rabbit IgG (H + L) NANOPARTICLE - Néodyme (Nd) WAVELENGTH - 340ex. – 880em.