PH analyzer SFR vario

densityoxygenCO2

The SFR vario offers online monitoring of oxygen, biomass and pH or CO2 - simultaneously. Online measured biomass data can be correlated with parameters like optical density, cell dry weight, or cell concentration. This way it is possible to get real-time information on e. g. OD600 development. The device optics can read out one oxygen and one pH or CO2 (CO2 Technical Data) sensor spots - integrated in the ready-to-use cultivation vessels - and also comprise a dedicated optical set-up for biomass monitoring. The oxygen uptake rate (OUR) can be calculated from the slope of the online oxygen measurements. The system has two long-lasting, rechargeable batteries, and is compatible with any standard shaking incubators. Up to 4 SFR vario can be controlled from the SFR vario software. Measurement data are transferred wirelessly via Bluetooth to a PC / notebook. Simultaneous real-time measurement of O2, biomass & pH or CO2 Automatic OUR calculation Online measurement of optical density, cell dry weight, cell concentration by correlation with biomass measurements Parallel measurements in up to 4 shake flasks Wireless data transfer enables easy integration Bioprocess development & media optimization APPLICATIONS Biomass, O2 & pH Monitoring during Growth of E. coli E. coli K12 shows distinct diauxic growth in medium containing glucose and lactose, which could be monitored with the SFR vario. Measurements were stopped several times for offline sampling - to determine substrate concentration - showing in gaps in the graphs. In the first growth phase glucose is consumed (1).