Carbon analyzer SFR

pHdissolved oxygenoxygen

The SFR Shake Flask Reader monitors pH, oxygen and OUR in up to 9 Erlenmeyer flasks, cultivation tubes, or T-flasks simultaneously. It fits in nearly all standard shakers. Measurement data is transferred wirelessly via Bluetooth to your PC / notebook. The corresponding ready-to-use vessels contain pre-calibrated sensor spots. The system monitors non-invasively through the transparent bottom of the container. Different types and sizes of flasks and tubes are available. Plastic, disposable flasks contain pre-calibrated oxygen and pH sensors, while re-usable glass flasks are equipped with autoclavable oxygen sensors. Fast monitoring of up to 63 shake flasks in parallel For microbial and cell culture Pre-calibrated cultivation vessels are ready-to-use Compatible with standard shakers Glass & plastic flasks in different sizes available Non-invasive measurement Used in seed train & bioprocess development APPLICATIONS Process Monitoring in Suspension-Adapted CHO Cell Cultures The online measurement of dissolved oxygen concentration and pH in shaken bioreactors paves the way for proper scale down activities from bench-top stirred-tanks to smaller scales. Adjustment of shaking speed as a function of pO2 is now possible avoiding possible oxygen limitations at high cell densities. Even a simple pH readjustment by tuning the pCO2 in the incubator is feasible to optimize the output from simple experiments with shaken bioreactors. Yeast & E. coli: Ensure Enough Oxygen Supply S. cerevisiae grows on different sugars as carbon source. While growth on glucose and fructose is mainly fermentative, growth on galactose is mainly respirative.