The FTC-PSt3-YAU is a miniaturized chemical optical oxygen sensor integrated in a flow-through cell. A glass tube with an inner diameter of 2 mm is coated with the sensor at its inner wall. The FTC is connected to the oxygen meter with an optical fiber. The volume for liquid inside the FTC is about 100 microliter. The standard flow-through cell can be easily connected via Luer-Lock adapters to external tubings.
Online oxygen measurements in perfusion systems
No consumption of oxygen
Signal independent of flow velocity
Autoclavable
APPLICATIONS
In-line Oxygen Monitoring in 3D Cell Culture
Since oxygen has a low solubility in culture medium it must be supplied to cells within 3D scaffolds via constant perfusion of the medium. The oxygen content of the medium before and after having passed by the cells can then help to determine whether the flow rate applied is sufficient. PreSens flow-through cells with integrated chemical optical sensors can be incorporated in the construct inlet and outlet and allow in-line measurement of oxygen tension.
Properties -
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Compatibility - Aqueous solutions, ethanol, methanol
No cross-sensitivity -
pH 1 – 14
CO2, H2S, SO2
Ionic species
Cross-sensitivity - Organic solvents, such as acetone, toluene, chloroform or methylene chloride
Chlorine gas
Sterilization procedure - Steam sterilization
Ethylene oxide (EtO)
Cleaning procedure - 3 % H2O2
Acidic agents (HCl, H2SO4), max. 4 – 5 %
Calibration - Two-point calibration with oxygen-free environment (nitrogen, sodium sulfite) and air-saturated environment
Storage stability - 24 months provided the sensor material is stored in the dark