Laboratory microplate OxoPlate OP96U

96-well

The OP96U is a round bottom 96-well multiplate with an oxygen sensor integrated on the bottom of each well. The plate is made of polystyrene. The sensors can be read out in parallel from the bottom side using a commercially available fluorescence reader. Ready-to-use User-defined batch calibration For use with conventional microplate fluorescence readers High throughput screening APPLICATIONS Enzyme Kinetics Measurement The OxoPlate OP96U can be used to determine enzyme activities. In this example kinetics of glucose oxidase (GOD) with different activities (0.05, 0.02, 0.01 and 0.05 U/mL) is shown. The OxoPlate filled with glucose solution and catalase (to avoid destruction of glucose oxidase by formed hydrogen peroxide) was incubated in the reader before the enzyme was added. Praffine oil was put into each well to reduce oxygen ingress. The oxygen measurement results show clearly the different dynamic equilibria of oxygen consumption and oxygen ingress depending on the added enzymes activity. TECHNICAL Specifications* * in H2O dest. or oxygen-free water Measurement range - 0 - 30 % O2 Resolution - ± 0.2 % O2 at 0.21 % O2 ± 0.6 % O2 at 20.9 % O2 Accuracy - ± 0.4 % O2 at 0.21 % O2 ± 1.0 % O2 at 20.9 % O2 Drift - < 0.04 % O2 per hour (sampling interval 1 min.) Measurement Temperature Range - from + 15 °C to + 45 °C Response time (t90) - at + 25 °C: < 30 sec. Properties Compatibility - Aqueous solutions, ethanol (max . 10 % v/v), methanol (max. 10 % v/v), pH 2 – 10, 20 % (v/v) DSMO, DMF, acetone, THF, or dioxane in solution. (Calibration constants have to be determined using the respective solvent instead of water.)