Laser-induced fluorescence detector PrinCE

for electrophoresisbenchtop

LASER Induced Fluorescence detectors are extremely powerful, typically capable of emitting light of a very well defined wavelength with an intensity of 1-40 mW. Thus the maximum possible number of molecules can be excited, resulting in the best possible limits of detection. For fluorescent compounds, concentration limits of detection down to 10-12 mol/l have been reported. Bearing in mind that typically about 10 nl of sample is injected, this equals about 10-20 mol! LIF detection can be more sensitive than any other commercially available detector, even if the compound is not fluorescent itself. Of course a suitable label should be available. Comparable with the LIF, LED is from 10–100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection. Low noise and interference molecules will be detected. Only labeled or native fluorescence can be measured.