Research assay kit Lumit™

cellimmunoassay

Fast add-and-read protocol performed directly on cells Luminescent detection with just a plate-reading luminometer Scalable to 96- or 384-well plates Quantify Cytokine Release Without Tedious Wash Steps The Lumit™ IL-4 Immunoassay quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate. Primary antibodies to IL-4 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of IL-4, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to IL-4 levels. Large Dynamic Range of Lumit™ IL-4 Detection The pg/ml to ng/ml linear range of Lumit™ detection covers expected concentrations for most cell models. Assay IL-4 Human Immunoassay Limit of Detection (LOD) 6.7pg/ml (3 SD above background) Dynamic Range 18.2pg/ml – 25ng/ml Minimal Detectable Dose (MDD) 4.5pg/ml (2 SD above background) Sample Type Cell culture supernatants Sensitive Quantitation of Low-Level IL-4 Release When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit™ IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.