Solution reagent kit DC6051

buffer solutionfor researchfor capillary electrophoresis

Quick preprocessing of bone powder using Demineralization Buffer Compatible with manual and Maxwell® DNA IQ™ chemistry Yields DNA free of inhibitors and compatible with downstream amplification reactions Note: The Bone DNA Extraction Kit replaces the custom version of the Bone DNA Extraction Kit . No changes have been made to the product composition, and therefore, no performance changes are expected during the transition of the product from the custom to catalog version. Rapidly Process Challenging Skeletal Remains In certain cases involving missing persons, mass disasters and anthropological excavations, bone and teeth are often the only sample types available for identification. Further, these samples are often subjected to environmental damage, and co-extraction of environmental inhibitors during purification limits their usability in STR typing experiments. Purification of DNA free of inhibitors is a crucial first step for obtaining a usable profile from these samples. A short preprocessing protocol followed by purification of DNA using DNA IQ™ chemistry yields inhibitor-free DNA compatible with PowerQuant® or Plexor® HY Systems for human and male DNA quantification. This DNA can be used with the PowerPlex® STR Systems to generate an STR profile. Bone DNA Extraction Method Overview In a 1.5ml microcentrifuge tube, 400µl of preprocessing buffer is added to bone powder and incubated at 56ºC for 2.5 hours on a shaker set at 1000rpm. The tube is then centrifuged for 5 minutes at 13,000 × g, and the supernatant is transferred to a new 1.5ml microcentrifuge tube containing Lysis Buffer with a reducing agent.