Medical research assay kit CellTox™ Green

cell viabilitycytotoxicitycell

Real-Time Cell Death Assay With Multiplexing Compatibility Accurate cytotoxicity determination in exposures out to 72 hours Flexible protocol allows kinetic analysis or endpoint determination Multiplex with luminescent assays to obtain more data per well Monitoring Cell Death in Real Time Using the CellTox™ Green Cytotoxicity Assay Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats. Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity Dose- and exposure-dependent increases in cytotoxicity In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding. Measure Cytotoxicity and Viability Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.