Medical research assay kit Caspase-Glo® 3/7

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High sensitivity caspase assay—requires fewer cells and less enzyme Simple add-mix-measure protocol—no sample preparation Easily scalable to 96-, 384- and 1,536-well plate formats Homogeneous Assay Measures Caspase-3/7 Activity The Caspase-Glo® 3/7 assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity. The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. High sensitivity allows use of the Caspase-Glo® 3/7 assay in 3D cell culture systems. The assay delivers excellent Z'-factor values in cell and purified enzyme models.