Medical research assay kit RealTime-Glo™

cell viabilitycytotoxicitycell

Monitor Cell Viability in Real Time Save time, cell culture and reagent costs Rapidly detect changes in cell viability Why Use the RealTime-Glo™ MT Cell Viability Assay? With the RealTime-Glo™ MT Cell Viability Assay, you can monitor cell viability continually in the same sample well up to 72 hours to obtain more information about the mode of action of a treatment with regard to time and dose dependence. The assay reagent can be added to cells during plating, during treatment or at the end of treatment. No cell washing, media removal or further additions are required. The nonlytic nature and rapid response of the assay make it possible to monitor cell viability over time in the same well, enabling easy multiplexing with other cell-based assays or downstream applications. The assay measures the reducing potential of viable cells and is ATP-independent, providing an orthogonal method for viability or cytotoxicity determination. Learn how the RealTime-Glo™ MT Cell Viability Assay can be used to assess drug toxicity in organoids. The RealTime-Glo™ Assay Measures Reducing Potential, A Well-Established Indicator of Cell Viability Assay Principle In the RealTime Glo™ MT Cell Viability Assay, a non-lytic NanoLuc® Luciferase reaction occurs in the culture medium. NanoLuc® Luciferase and MT Cell Viability Substrate are added to cell culture media. The MT Cell Viability Substrate then diffuses into cells where it is reduced to form a NanoLuc® Substrate, which exits the cell and is used rapidly by NanoLuc® Luciferase in the media. Only metabolically active cells can reduce the substrate, and light production is proportional to the number of live cells in culture.