Medium reagent FuGENE®

for researchfor molecular biologyfor nucleic acids

Difficult-to-transfect cell lines Transfection of primary cells Protein and virus production Use the Best Cell Line for Your Study FuGENE® HD Transfection Reagent offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al. [2009] Biotechniques 47, 617–24). It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production. FuGENE® HD gives more biologically relevant results by allowing you to use the best cell line for your study, and doesn’t require harsh treatment of the cells by lowering serum concentration or washing of toxic lipid complexes. The in vivo environment even can be mimicked with transfection in 100% serum. High Expression with Minimal Effect on Cell Health This figure compares transfection reagent performance in HEK293 cells. Cells were transfected with a luciferase reporter plasmid, and luciferase activity detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. FuGENE® HD Transfection Reagent gave the highest expression levels and lowest toxicity.