Standard overnight digestion, nondenaturing conditions
Increased peptide recovery, enhanced protein quantitation, improved reproducibility
Tolerant to trypsin-inhibiting contaminants
Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing
Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.