Enzyme reagent V50 series
for researchfor mass spectrometryprotein

enzyme reagent
enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for research, for mass spectrometry
Tested parameter
protein
Storage temperature

Max.: -10 °C
(14 °F)

Min.: -30 °C
(-22 °F)

Description

Standard overnight digestion, nondenaturing conditions Increased peptide recovery, enhanced protein quantitation, improved reproducibility Tolerant to trypsin-inhibiting contaminants Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation. Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system. Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.

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