DNA polymerase reagent GoTaq® G2

antibodyTaq DNA polymerasefor research

Hot-start Taq eliminates nonspecific PCR amplification Detects low-copy-number targets Allows optimization of reactions with Flexi Reaction Buffer and separate MgCl2 Supplied at a concentration of 5u/μl Hot Start Taq with Superior Performance, Improved Yield, Greater Sensitivity GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience. GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.