Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
Resistant to most PCR inhibitors
Simple, Sensitive Probe-Based qPCR and RT-qPCR
The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These real-time qPCR systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.
Rapid hot-start activation and processive enzymes make the GoTaq® Probe qPCR and RT-qPCR Systems compatible with most real-time PCR instruments using standard or fast cycling. Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.
Sensitivity and Specificity for qPCR
The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.
The GoTaq® Probe qPCR Master Mix offers:
Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
Optimized formulation compatible with running multiplex assays.