Enzyme reagent GoTaq®

dyefor researchfor PCR

Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR Compatible with both fast and standard cycling methods Resistant to a wide range of PCR inhibitors Simple, Sensitive, Dye-Based qPCR and RT-qPCR The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I. Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR. Sensitivity and Specificity for qPCR The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR. Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers: Sensitive detection for earlier quantitation of low- and high-copy targets. Room-temperature setup,making it suitable for automation and high-throughput detection. Compatibility with both fast and standard qPCR cycling methods.