Serum reagent kit Ester-Glo™

tissuelipoproteinlipid

Works with lysed cells, tissues, medium and serum samples No organic extraction, extreme heat or centrifugation required Linear cholesterol detection up to 80µM Sensitive, Rapid Detection of Cholesterol and Cholesterol Esters from a Variety of Samples The Cholesterol/Cholesterol Ester-Glo™ Assay is a bioluminescent assay for rapid and sensitive method for measuring cholesterol and cholesterol esters in cultured cell lysates and other biological samples, such as lipoprotein fractions, cell culture medium, serum and tissue homogenates. Cholesterol is an essential lipid involved in steroidogenesis, bile acid synthesis, cell signaling and maintenance of membrane structure. How the Assay Works The Cholesterol/Cholesterol Ester-Glo™ Assay measures cholesterol using a cholesterol dehydrogenase that links the presence of cholesterol to the production of NADH to the activation of a proluciferin that produces light with luciferase.In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP, and the amount of light produced is proportional to the amount of cholesterol in the sample. For measuring Cholesterol Ester, the assay includes a cholesterol esterase that will remove the fatty acid from cholesterol esters to produce one molecule of cholesterol per molecule of ester. The amount of cholesterol esters is determined from the difference of cholesterol measured in the absence (free cholesterol) and presence (total cholesterol) of esterase.