Solution reagent kit Wizard® HMW

blood samplefor DNA extractionfor DNA sequencing

Optimized protocol for extracting high-purity HMW DNA Reliable performance on long-read systems such as the Oxford Nanopore Technologies® instruments Rapid protocol with no hazardous chemicals High-Quality High-Molecular-Weight DNA from a Variety of Sample Types De novo genome sequencing, genome finishing and structural variant detection have become significantly more commonplace due to advances in sequencing technology. Long-read sequencing in particular provides researchers with data to address challenges in fields such as oncology, inherited disease research, microbiology and agriculture. However, long-read technologies require high-quality, high-molecular-weight DNA. Obtaining DNA of sufficient, size, quantity and quality is critical to the process that has historically required costly or laborious methods. The Wizard® HMW DNA Extraction Kit is specifically designed to isolate high-molecular-weight (HMW) DNA that will provide strong performance in long-read sequencing. This kit helps researchers obtain DNA up to 500kb with high purity to get the most out of precious samples and can be used with a wide range of sample types, including whole blood, plant leaf, tissue culture cells and Gram-positive and Gram-negative bacteria. With a workflow that can be completed in approximately 1.5 hours without specialized equipment, the Wizard® HMW DNA Extraction Kit relieves many of the challenges regularly faced by researchers. HMW DNA in Long-Read Sequencing Samples of human blood and cultured K562 mammalian cells were used to extract high-molecular-weight DNA using the Wizard® HMW DNA Extraction Kit.