JAK2 mutation test kit ipsogen

for researchoncologygenomic DNA

Sensitive quantitative assay Accurate quantitation of the JAK2 V617F/G1849T mutation Easy workflow with ready-to-use solutions Product Details The ipsogen JAK2 MutaQuant Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantitation of the JAK2 V617F/G1849T mutation in genomic DNA. Performance To ensure highest sensitivity, the ipsogen JAK2 MutaQuant Kit has been optimized to detect the JAK2 V617F/G1849T mutation and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of JAK2 V617F plasmid standards and Reliable detection of JAK2 wild-type plasmid standards). The sensitivity has a limit of detection (LOD) of 0.061%. This optimal sensitivity can be obtained on specimens containing at least 10,000 copies of the JAK2 gene (wild-type and V617F mutation). Principle The ipsogen JAK2 MutaQuant Kit is a ready-to-use kit for the detection and quantification of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure JAK2 mutation site identification). The kit provides high levels of sensitivity and reproducibility. The ipsogen JAK2 MutaQuant Kit provides 4 plasmid standard dilutions for mutant JAK2 V617F and 4 for the wild-type JAK2 allele. Use of the standards enables accurate quantification of transcripts. Results are expressed as a percent of JAK2 V617F in total JAK2. Procedure The first step is extraction and determination of quality and quantity of genomic DNA.