Research assay kit 222115

for molecular diagnosticviruscoronavirus

targets ORF1a, ORF1b, ORF3a, ORF7a, N1 and N2 within the SARS-CoV-2 genome Compatible with nasopharyngeal and oropharyngeal swabs, lolly (lollipop swab), gargle and saliva Detects six different targets in three color channels (FAM, ROX and Cy5) Does not detect SARS-CoV-1 or other Sarbecovirus species Ready-to-use assay mix (all-in-one tube) Product Details To ensure future-proof detection of SARS-CoV-2, we developed a novel assay kit covering six targets across four genomic regions (ORF1ab, ORF3a, ORF7a and N gene) of the virus. Using multiple targets in multiple regions increases the likelihood of successful detection. Probes are coupled to different dyes and can be measured in the FAM, ROX and Cy5 channels on a cycler of your choice. This kit can be used in combination with the QIAprep& Viral RNA UM Kit chemistry and SARS-CoV-2 Neo Positive Control to provide fast and easy detection. Sample types compatible for detecting SARS-CoV-2 include nasal, nasopharyngeal, oropharyngeal swabs, lolly (lollipop swab), saliva, and gargles. Performance The unique design of the SARS-CoV-2 Neo Assay enables reliable and highly specific SARS-CoV-2 detection. In silico analyses showed that all investigated SARS-CoV-2 viral sequences (>4.8 Mio) were successfully detected by this assay. The assay does not detect SARS-CoV-1, other Sarbecovirus species, and/or other organisms as demonstrated by wet-lab and in-silico analyses. The list of wet-lab-tested respiratory pathogens includes Influenza A (H3N2, H1N1), Influenza B, Parainfluenza (1-4), Coronavirus 229E, OC43, NL63, C. pneumoniae, Metapneumovirus,B. pertussis, Human Rhinovirus 1A, RSV A, Human Adenovirus 5, L.