For the quantification of BCR-ABL1 Mbcr b3a2 and b2a2 fusion gene transcripts, results alignment on the international scale, MMR and MR4.5 reporting
The BCR-ABL1 Mbcr RGQ RT-PCR Kit is intended for research use only and is not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Features
MMR and MR4.5 reporting
Results aligned on the International Scale (IS)
With an IS-MMR calibrator in every run to prevent intra- and inter-lab variation
High reliability using EAC technology and single plasmid standards
Ready-to-use solutions including all reagents for RT and RQ-PCR validated on Rotor-Gene Q 5Plex HRM
Product Details
Performance
To ensure the highest sensitivity, BCR-ABL1 Mbcr RGQ RT-PCR Kit has been optimized to detect very low levels of BCR-ABL1 p210 b2a2 or b3a2 transcripts. The limit of detection (LOD) is 3.13 CNMbcr (corresponding to a LOD of 0.0031% with ABL1CN = 100 000).
Principle
BCR-ABL1 Mbcr RGQ RT-PCR Kit is a ready-to-use kit for the detection of BCR-ABL1 p210 b2a2 or b3a2 transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL1 p210 b2a2 or b3a2 transcripts, relative to ABL1 control gene expression, in total RNA. The kit provides high levels of specificity, sensitivity, and reproducibility. Each kit provides 4 standard dilutions for ABL1, and 5 standard dilutions for BCR-ABL1 Mbcr. Use of a single plasmid for BCR-ABL1 and ABL1,