RT-PCR reagent kit QuantiTect

reverse transcriptasefor cDNA synthesisgenomic DNA

The QuantiTect Reverse Transcription Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. Features cDNA synthesis and gDNA removal in only 20 minutes High cDNA yields even from low-abundance transcripts cDNA synthesis from 5' and 3' regions of transcripts No need to design RNA-specific primers or probes Product Details The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect Reverse Transcription Kit, including Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, and a unique RT Primer Mix. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts. Performance Using the QuantiTect Reverse Transcription Kit, contaminating genomic DNA in RNA samples is effectively and rapidly removed with the unique gDNA Wipeout Buffer (see figure Effective genomic DNA removal for accurate real-time RT-PCR). Elimination of genomic DNA is crucial for accurate gene expression results, and design of RNA-specific primers or probes is not always possible. With gDNA Wipeout Buffer, time is saved and costs are reduced, since a separate DNase digestion is unnecessary, either during or after purification of RNA samples. The high RNA affinity of Quantiscript Reverse Transcriptase, in combination with Quantiscript RT Buffer,